PerkinElmer
check quantity

Streptavidin AlphaLISA® Acceptor beads, 250 µg

AlphaLISA® Acceptor beads conjugated to streptavidin. These beads can be used to capture biotinylated proteins, peptides, nucleic acids (DNA and RNA), and small molecules for AlphaLISA no-wash assays.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Part Number Unit Size List Price Your Price Quantity
AL125C 250 µg 571.00 USD
AL125M 5 mg 5700.00 USD
AL125R 25 mg 23400.00 USD
Buy Now

You successfully added item(s) to your cart

Overview

These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA® no-wash assays for:

  • Protein-protein interaction assays
  • Protein-DNA interaction assays
  • Protein-RNA interaction assays
  • Protein-small molecule interaction assays
  • Analyte detection assays
  • Biomarker detection assays
  • Antibody detection assays
  • Enzymatic assays
  • Other immunoassays

In a typical AlphaLISA assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume.

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugates Streptavidin
Automation Compatible Yes
Bead Type or Core Bead Type AlphaLISA Acceptor
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Unit Size 250 µg

Resources, Events & More

All (16)
Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Brochure Handle Large Biomolecular Interactions With Ease PDF  798 KB
Guide Alpha Protein-Protein Interaction Quick Start Guide PDF  380 KB
Guide AlphaLISA immunoassay development quick guide PDF  1 MB
Guide ELISA to AlphaLISA Immunoassay Conversion Guide PDF  1 MB
Guide User's Guide To Alpha Assays Protein:Protein Interactions PDF  3 MB
Application Note Biochemical Binding ADCC Assays Utilizing AlphaLISA Toolbox Reagents PDF  1 MB
Application Note Developing Novel Tetraubiquitin Substrates and AlphaLISA Technology to Provide Next Generation Deubi PDF  2 MB
Application Note Development and Utilization of Bromodomain and Extra Terminal Domain (BET) Assays Using AlphaLISA PDF  1 MB
Application Note Development of Pharmacokinetic (PK) Assays for Detecting Biosimilars Targeting TNFa Using AlphaLISA PDF  2 MB
Application Note Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction PDF  3 MB
Technical Note Designing Your Own AlphaLISA Assay: Selecting Toolbox Bead Pairs to Avoid Potential Bead-Bead Intera PDF  1 MB
Poster AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices PDF  273 KB
Poster AlphaLISA: The Fast and Easy Non-Wash Alternative to ELISA PDF  793 KB
Poster Rapid, Homogeneous and Robust HIV-p24 Detection Assay Using the AlphaLISA Platform PDF  318 KB
Event Society of Laboratory Automation & Screening (SLAS) Event