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LANCE Eu-W8044-DTA Chelate and Eu Standard, 1 mg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling.

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AD0020 12300.00 USD

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Overview

LANCE® Eu-W8044-DTA Chelate is optimized for the labeling of proteins and peptides containing at least one primary aliphatic amino group. The labeled compound can be used in LANCE® homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assays and fluorescence quenching assays (TF-FQA).

  • More stable; can be used in assays with concentration of EDTA up to 250 mM (under pH neutral conditions), or when the pH of your assay is low (down to pH 4).
  • In LANCE assays, APC (allophycothiocyanin) or ULight-labeled compounds can be used as an acceptor. 
  • Dichlorotriazine activated chelates, are suitable for labeling proteins, peptides, nucleic acids and small molecules containing at least one primary aliphatic amino group.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC)]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W8044-DTA
Product Brand Name LANCE
Shipping Condition Blue Ice
Unit Size 1 mg