Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.
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LANCE® Eu-W1024-ITC Chelate is optimized for the labeling of proteins and peptides at least one primary aliphatic amino group. The labeled compound can be used in homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assays (LANCE® assays).
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC)]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE|
|Shipping Condition||Blue Ice|
|Unit Size||1 mg|
|Resource Type||File Name||File Format|
|Guide||LANCE Protein: Protein Interaction Quick Start Guide||PDF 1 MB|
|Guide||LANCE TR-FRET protein-protein interaction assay development guide||PDF 1 MB|
|Manual||LANCE Eu-W1024 ITC Chelate & Europium Standard AD0013||PDF 82 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|