Uncoated SPA beads that interact with primary phosphate groups in nucleotides (oligos, DNA and RNA); membranes can also bind.
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SPA Scintillation beads are microspheres containing scintillant which emit light in the blue region of the visible spectrum. As a result, these beads are ideally suited to use with photomultiplier tube (PMT) counters such as the MicroBeta2 or TopCount.
Two types of core SPA Scintillation bead are available - yttrium silicate (YSi) and Polyvinyltoluene (PVT). PVT beads are plastic, larger in size, and stay in suspension longer than the crystalline YSi beads.
Scintillation proximity assay (SPA) is a homogeneous and versatile technology for the rapid and sensitive assay of a wide range of biological processes, including applications using enzyme and receptor targets, radioimmunoassays, and molecular interactions. When 3H, 14C, 33P, and 125I radioisotopes decay, they release β-particles (or Auger electrons, in the case of 125I). The distance these particles travel through an aqueous solution is dependent on the energy of the particle. If a radioactive molecule is held in close enough proximity to a SPA Scintillation Bead or a SPA Imaging Bead, the decay particles stimulate the scintillant within the bead to emit light, which is then detected in a PMT-based scintillation counter or on a CCD-based imager, respectively. However, if the radioactive molecule does not associate with the SPA bead, the decay particles will not have sufficient energy to reach the bead and no light will be emitted. This discrimination of binding by proximity means that no physical separation of bound and free radiochemical is required.
|Bead Type or Core Bead Type||YSi|
|Coating Treatment||RNA Binding|
|Product Brand Name||SPA Scintillation Beads|
|Unit Size||500 mg|
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|Resource Type||File Name||File Format|
|Application Note||Assay Development Guide to Protein:Protein Studies||PDF 951 KB|
|Application Note||The Application of SPA Technology to Study Protein: DNA Interactions||PDF 997 KB|
|Application Note||The Application of SPA to SH2 and SH3 Domain Binding to Specific Peptide Sequences||PDF 1 MB|
|Poster||Miniaturization of the Phosphodiesterase SPA Assay||PDF 206 KB|
|Poster||Monitoring Activation at G-Protein Coupled Receptors with Functional Screening Assay Systems||PDF 1 MB|
|Poster||The Use of Fluorescence Technologies to Measure Specific Signal Transduction Events and Molecular Interactions||PDF 135 KB|
|Poster||Using High Throughput SPA to Screen for CYP2D6 Interactions||PDF 106 KB|