Lentiviral particles for tranduction of mammalian cell lines with a red-shifted luciferase transgene, for use in in vivo and other applications.
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RediFect™ Red-FLuc-Puromycin Lentiviral Particles are self-inactivating, recombination incompetent lentiviral particles carrying red-shifted Luciola Italica luciferase transgene under control of the stable UbC promoter. The luciferase transgene is fused to the puromycin resistance gene via T2A “self-cleaving” linker peptide for efficient co-expression with selection marker. The lentiviral particles are pseudotyped with G glycoprotein from Vesicular Stomatitis Virus (VSVG), allowing efficient transduction of a wide variety of mammalian cells including most cancer cell lines, primary, stem and non-dividing cells.
We recommend using this product to transduce human cell lines, or murine cell lines that will be used in Nude and SCID mouse models only. An immune response may occur in syngeneic mouse models.
Disclaimer: For laboratory use only. This product is intended for animal research only and not for use in humans.
|Optical Imaging Classification||Bioluminescence Imaging|
|Product Brand Name||RediFect|
|Shipping Condition||Dry Ice|
|Unit Size||1 Vial|
|Resource Type||File Name||File Format|
|Brochure||In Vivo Imaging Agents Brochure||PDF 2 MB|
|Technical Note||RediFect Red-FLuc-Puro Lentiviral Particle||PDF 327 KB|
|Specification Sheet||RediFect Lentiviral Particles Transfection Protocol||PDF 91 KB|
|Citations Library||View peer reviewed publications using PerkinElmer's In Vivo Imaging Reagents||Link|
|Webinar||View our complete list of in vivo webinars available on demand||Link|