Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
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AequoScreen® Double Transfected Cell Line: Human recombinant P2Y11 receptor in aequorin 1321N1 host cell. We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant receptor in a functional assay. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
Disclaimer: This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
|Assay Target Class||GPCR|
|Assay Target Type||Cell line|
|Assay Validated||Calcium Luminescence|
|G-Alpha Natural Receptor||Gq/G11|
|Product Brand Name||AequoScreen|
|Receptor Type||Purinergic P2Y|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Therapeutic Area||Metabolic, Inflammation|
|Unit Size||5 million cells|
|Resource Type||File Name||File Format|
|Brochure||EnSpire Multimode Plate Reader with Label-free Technology Brochure||PDF 4 MB|
|Application Note||AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines||PDF 607 KB|
|Application Note||Aequorin bioluminescence assays on MicroBeta2 LumiJET for studying Ca2+-coupled GPCRs and ion channels||PDF 152 KB|
|Application Note||Pathway independent cell-based assays on the EnSpire Multimode Plate Reader with Epic® label-free technology||PDF 1 MB|
|Flyer||Aequorin FLIPR||PDF 1 MB|