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Operetta High-Content Imaging System

As scientists grapple with increasingly complex biological questions, they need to conduct larger, more complex imaging experiments. So they’re turning to the Operetta® High Content Imaging System – the ideal solution for analyzing the inner workings of healthy and diseased cells.

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Introducing the New Operetta CLS™ High-Content Analysis System

Whether you are performing assay development, genome-wide siRNA, compound screens or looking at a few samples in great detail, and whether you are using 3D disease models, primary cells, stem cells, live or fixed cells, the Operetta High Content Imaging System delivers all the features you need to generate unbiased, statistically significant data to move your research forward.

And it’s so simple to use that even newcomers to high content imaging and high content analysis can be productive right away.

See your research in stunning clarity

  • Fast widefield imaging for routine applications
  • True spinning disk confocal imaging option to eliminate background, and protect live cell samples by minimizing photobleaching and phototoxicity
  • Spinning disk pinhole size optimized for good confocality across a wide range of objectives
  • Equipped with 8 position dichroic wheel for maximum excitation flexibility
  • Unsupervised automated image acquisition and autofocus for reliable high content imaging from slides to multiple plates
  • Digital Phase contrast for imaging and segmentation of cells that have not been fluorescently labeled
  • High numerical aperture objectives give exceptional images for higher quality data and more reliable results
  • Low and high magnification plus a large field of view allows you to look at the whole well and choose an area of interest for detailed analysis
  • Get better results from your Operetta system with our microplates for high content imaging, such as the CellCarrier Ultra 384-well microplates designed for optimal performance in high content imaging applications and our CellCarrier Spheroid ULA microplates for imaging 3D models
  • Expert, global service and applications support from our dedicated team of lab- and field-based application specialists, plus an exclusive HCS support portal

Say Hello to Harmony and Goodbye to Bias

  • Intuitive Harmony® High Content Imaging and Analysis Software makes it easy for biologists to turn their biology into unbiased quantitative data
  • Developed based on many years of experience in high content analysis by our expert team with regular updates
  • Easily set up assays, automate experiments, analyze data and store, retrieve and present results
  • Intuitive plate wizard for even simpler set up of new plate types - automatically detects the important parameters plate bottom height and plate bottom thickness
  • Analysis templates for a range of common applications get new users productive with high content analysis right away
  • User-configurable image analysis building blocks for easy creation of customized high content analysis applications
  • STAR analysis provides a more detailed analysis of cellular morphology for accurate differentiation of cellular phenotypes
  • Texture analysis for applications where intensity and morphology are not suitable or do not deliver robust readout and reproducible results
  • Transfer and view data from any computer that has an installation of the Harmony software
  • Cell tracking lets you see temporal changes of biological systems and study cell dynamics in 2D
  • NEW: Advanced building blocks for easier analysis of complex applications such as FRET
  • NEW: Improved memory management for quicker processing – up to 3 times faster than Harmony 3.5
  • New: XYZ View shows an xy-, xz- and yz-section through an image stack of a thick sample e.g. microtissues

Flexibility and ease for all your applications

  • Suitable for a wide range of fixed and live cell applications
  • Live cell chamber option to maintain environmental conditions (temperature, CO2) to protect your live cell samples
  • Kinetic capabilities for carrying out time course experiments for a better understanding of cellular dynamics
  • Integrates with our robotic automation platforms for a fully automated solution.

Assay Groups

Whichever assay you are working on, the Operetta High Content Imaging System offers a solution:

  • Apoptosis
  • Cell Cycle
  • Cell Differentiation
  • Cell Migration
  • Cell Proliferation
  • Cell Shape Changes
  • Cytoskeletal Rearrangement
  • Cytotoxicity
  • Fluorescence in situ hybridization (FISH)
  • Lipid Droplet Analysis
  • Neurite Outgrowth
  • Protein Expression
  • Receptor Activation
  • RNAi Screening
  • Signaling Pathway Analysis
  • Transcription Factor

Visit our support portal

Disclaimer:  For research use only. Not for use in diagnostic procedures.


21 CFR Part 11 Compatible No
Automation Compatible Yes
Detection Method Transmission, Fluorescence
Interface Ethernet
Light Source Xenon
Portable No
Product Brand Name Operetta
Warranty Yes

Resources, Events & More

All (41)
Resource Type File Name File Format
Brochure 3D Cell Culture Solutions Brochure PDF  4 MB
Brochure Drug Discovery Screening Solutions Brochure PDF  880 KB
Brochure Operetta High-Content Imaging System Brochure PDF  4 MB
White Paper Combining Promega HaloTag® Technology with PerkinElmer High Content Imaging Systems PDF  1 MB
Application Note 3D Analysis of Cell Invasion using Operetta PDF  1 MB
Application Note A Multiparametric Live-Cell Cytotoxicity Analysis using the Operetta PDF  1 MB
Application Note Analysis of Stem Cell Differentiation using Operetta PDF  1 MB
Application Note Automated Single Cell Tracking using Operetta PDF  2 MB
Application Note Comparison of HCA ImagAmp and Conventional Indirect Immunofluorescence for High-Content Analysis Applications PDF  508 KB
Application Note Cytotoxicity Studies on 3D Primary Liver Microtissues PDF  2 MB
Application Note Cytotoxicity Studies on Live Primary Human Hepatocytes using Operetta PDF  2 MB
Application Note Determination of Cell Cycle Phase using the Operetta PDF  618 KB
Application Note Image-based Quantification of PKC Activation using Operetta PDF  827 KB
Application Note Image-based Quantification of the Huntingtin Protein using the Operetta PDF  833 KB
Application Note Quantitative Analysis of 3D Microtissue Growth and Biomarker Intensity PDF  916 KB
Application Note Quantitative Analysis of Embryoid Bodies Using the Operetta System PDF  921 KB
Application Note Screening Epigenetic HDAC Inhibitors – A Workflow Comprising HTS and HCS PDF  1 MB
Application Note Signal Amplification Strategies for Rapid and Sensitive High Content Analysis PDF  776 KB
Application Note Studying Kidney Injury using Operetta PDF  1 MB
Application Note Studying NfkB signaling dynamics using Operetta PDF  864 KB
Application Note Visualization of Cathepsin Activity using Operetta and ProSense 680 PDF  1 MB
Technical Note Workflows for In-depth High Content Analysis PDF  1 MB
Poster Automation of both sample preparation and analysis of a cell migration assay PDF  911 KB
Poster Bioluminescence Resonance Energy Transfer for High-Content Imaging PDF  1 MB
Poster Chromobody® based High Content cell cycleanalysis in live cells PDF  666 KB
Poster Enhanced assay sensitivity with HCA ImagAmp™: An enabling signal amplification high content technology for technically challenging targets. PDF  665 KB
Poster High content analysis of biomarker intensity and distribution in 3D microtissues using the Operetta® High Content Imaging System PDF  1 MB
Poster Image-based quantification of HCS cell-based assays using the Operetta / Harmony imaging platform PDF  867 KB
Poster Measuring of migration in a fully automated imaging based approach PDF  1 MB
Poster Quantification of NF-kB signaling in living cells:A high-content workflow for the generation oftime-resolved single-cell data PDF  589 KB
Poster Reagent-based Signal Amplification for High Content Analysis of PKCa Localization in HeLaCells. PDF  801 KB
Poster Screening HDAC Inhibitors – A Workflow Comprising High Throughput And High Content Screening PDF  720 KB
Poster Studying kidney injury using the Operetta High Content Imaging System PDF  454 KB
Featured Publication Note Tobacco-specific Carcinogen-operated Calcium Channel Promotes Lung Tumorigenesis PDF  217 KB
Case Study B Cell Selection and Therapeutic Antibody Characterization using Operetta PDF  1 MB
Case Study Inhibition of MicroRNA Sensitizes 3D Breast Cancer Microtissues to Radiation Therapy PDF  786 KB
Case Study A Workflow to Characterize and Benchmark Human Induced Pluripotent Stem Cells Link
Case Study High-Content Analysis of Drug-Induced Oligodendrocyte Differentiation Promoting Remyelination in Multiple Sclerosis Link
Link For comprehensive information about our products, technologies and software for high-content screening and analysis, please visit our microsite Link
Webinars Quantification of NF-kB Signaling in Living Cells using Operetta system and Harmony software Link
Webinars Automated Single Cell Tracking using Operetta system and Harmony software Link