PerkinElmer
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Operetta CLS High-Content Analysis System

Uncover deep biological understanding in your everyday assays and innovative applications using the Operetta CLS high-content analysis system. Featuring a unique combination of technologies, the system delivers all the speed, sensitivity and resolution you need to reveal fine sub-cellular details. And with our simple, powerful Harmony 4.5 software, Operetta CLS lets you find even subtle phenotypic changes.

Part Number HH16000000
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For research purposes only, not intended for diagnostic use

Detail Information

The Operetta CLS system combines speed and sensitivity with the powerful and intuitive data analysis you’ve come to trust from the Operetta platform. The all-new Operetta CLS delivers everything you need from high-content analysis. What’s more, the Operetta CLS system is part of our comprehensive HCS workflow – everything from HCS systems and microplates to automation and informatics for every application. All from one knowledgeable, trusted vendor. Put that together with our Harmony® high-content imaging and analysis software – the easy-to-learn, easy-to-use software that empowers biologists to do their own analysis – and you have everything you need to run your everyday (and complex) analyses right away.

A Unique Combination of Technologies

At the core of the Operetta CLS high-content analysis system is a new light path that ensures efficient excitation of your samples and careful collection of emitted signals.

  • Powerful, stable 8x LED light source for optimal excitation of your fluorophores and confidence in results
  • Proprietary automated water-immersion objectives with very high numerical aperture enable high resolution and fast read times with minimal photodamage
  • Confocal spinning disk technology provides a fast and gentle imaging process, enabling efficient background rejection, live cell experiments, and 3D imaging
  • Large format sCMOS camera delivers low noise, wide dynamic range, and high resolution – perfect for sensitive and quantitative measurements at short exposure times
  • User accessible bar-coded emission filters provide flexibility, sensitive imaging and error-free operation
  • Simple, powerful Harmony 4.5 high-content imaging and analysis software for easy set-up of assays, automation of experiments, image acquisition, data analysis, and storage and retrieval of results

A Solution Configured To Suit Every Need;

Whatever your application, there’s an Operetta CLS system configured to meet your requirements. And it’s modular, so it can change with your research demands. Several configuration options are available, Typical configurations include:

Operetta CLS Quattro

  • With four LEDs and widefield fluorescence, the basic configuration of the Operetta CLS system is ideal for common applications that need sensitivity and resolution, with the capacity to grow if the need arises.

Operetta CLS FLEX

  • With eight LEDs, and confocal and widefield fluorescence, the FLEX configuration offers flexibility in excitation and imaging modes for many challenging applications – and it can be upgraded to even higher performance.

Operetta CLS LIVE

  • With all the features of the FLEX configuration, plus gas and temperature control and water-immersion objectives, this system is ideal for gentle yet highly sensitive live cell imaging.

Applications

From everyday assays to more demanding applications, the Operetta CLS high-content analysis system delivers just the right combination of flexible excitation, sensitive optics, and advanced software features to enable you to gain deeper biological insight from all your critical applications.

Fixed-cell assays

  • Up to eight high-power excitation sources and user-accessible emission filters enable maximum flexibility of fluorescent stains and labels, plus the system features widefield and spinning-disk fluorescent imaging.

Live-cell assays

  • The spinning disk confocal optics and synchronized LED illumination provide stable excitation and minimize phototoxicity and bleaching for meaningful live-cell assays. You can also choose brightfield or digital-phase contrast imaging modes.

Complex cellular models

  • The large-format sCMOS camera with water-immersion objectives provides sensitivity and high resolution, while advanced software helps you address the imaging and analysis challenges presented by complex cellular models.

Advanced assays

  • FRET is a powerful tool for investigating conformational changes and protein-protein interactions. The Operetta CLS system’s sensitive imaging and dedicated analysis tools for ratiometric imaging, facilitate robust results.

Phenotypic fingerprinting

  • The Operetta CLS combines high resolution imaging with advanced software tools to help create robust phenotypic fingerprints of the subtle differences at the core of successful phenotypic assays.

Specifications

21 CFR Part 11 Compatible No
Automation Compatible Yes
Detection Method Transmission, Fluorescence
Height 45.0 cm
Imaging Modality High Content
Interface Ethernet
Length 98.0 cm
Light Source LED
Portable No
Product Brand Name Operetta CLS
Warranty Yes
Width 66.0 cm
Resources, Events & More
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Application Note

Analyzing ERK Signal Transduction in Live Cells Using a FRET-Based Biosensor

Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This application note describes how a live cell FRET assay to study ERK signaling was performed on the Operetta CLS™ high-content analysis system. The optimized design of the FRET-based biosensor, the high-quality imaging of the Operetta CLS system and the easy-to-use image analysis tools of the Harmony® software contribute to the robustness of the high-content assay.

PDF 1 MB
Automated Single Cell Tracking using the Operetta System: Analyzing Chemokinesis of Cancer Cells

In this application note, we describe a high-content screening application for analyzing the migration of non-small cell lung cancer cells in a live cell assay. Using the Operetta® high-content imaging system and digital phase contrast imaging, we tracked migrating cancer cells using automated single cell tracking in the Harmony® high-content imaging and analysis software.

PDF 1 MB
Distinguishing Cell Types by Phenotypic Profiling of the Nucleus

The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties. As nearly all screening approaches require a nuclear counterstain to facilitate segmentation, phenotypic profiling of the nuclei can offer new and additional perspectives on assays at no extra cost. This study shows how a single nuclear stain can enable phenotypic profiling and how phenotypic profiles can be used to distinguish up to seven different cell types, without further staining or phenotypic markers. Such methods could be applied to other fluorescent labels, opening up new horizons for unbiased drug discovery and disease research.

PDF 1 MB
Phenotypic Analysis of Hypertrophy in Human iPSC-Derived Cardiomyocytes

Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assay, the Opera Phenix system is 4 times faster than the previous Opera® system. Primary neuron morphology is analyzed in a straightforward approach using Harmony software. Careful assay optimization can increase throughput, and minimize the data burden, without compromising assay performance.

PDF 1 MB

Article

5 Top Tips for a Successful High-content Screening Assay with a 3D Cell Model System

Whether you’re familiar with high-content screening and are looking to exploit the increased physiological relevance of complex 3D cell models, or you want to take your analysis of 3D cell models to the next level, migrating from simple plate-reader assays to a high-content approach, you’ll need the right tools and strategies to overcome the challenges these models present.

PDF 1 MB

Brochure

With 3D Cell Cultures Your Science Comes Alive

Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cell

PDF 4 MB

Case Study

A Workflow to Characterize and Benchmark Human Induced Pluripotent Stem Cells

Human induced pluripotent stem cells (iPSCs) offer tremendous opportunities for disease modeling and discovery of novel therapeutics. The UK-based Human Induced Pluripotent Stem Cell Initiative (HipSci) aims to advance iPSC technology and pave the way to discovering how genomic variation impacts cellular phenotype by offering the scientific community access to a vast panel of cell lines with thorough characterization and data analysis tools. This case study details a phenotypic screen used to characterize human iPSCs on diverse extracellular matrix substrates, and a method for the capture of specific phenotypes emerging upon cell-to-cell contact.

PDF 1 MB
High-Content Analysis of Drug-Induced Oligodendrocyte Differentiation Promoting Remyelination in Multiple Sclerosis

One of the greatest challenges in multiple sclerosis (MS) therapy is the halting or reversal of the failure of remyelination in the brain in order to reverse disabilities in MS patients. This case study highlights the recent work of Dr. Paul Tesar and colleagues at the Case Western Reserve University School of Medicine, which could potentially lead to such novel treatments, as it aims to control the function of stem cells in the body and thereby to help the body repair itself.

PDF 3 MB
High-Content Analysis: Advancing Your Knowledge to Help Combat Infectious Disease

Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches. In our e-book, learn how high-content imaging and analysis plays a significant role in infectious disease research.

PDF 4 MB

Manual

Five Top Tips for a Successful High-Content Screening Assay with a 3D Cell Model System

Whether you’re familiar with high-content screening, or a newcomer, you’ll need the right tools and strategies to overcome the challenges of using complex 3D cell models in such an assay. For example, growing consistent, reproducible 3D cultures can be problematic and imaging large, thick cell samples can be challenging, while managing the huge volumes of data generated is perhaps the most demanding aspect of all. In this article, we provide our top tips for running a successful high-content screening assay using a 3D cell model. Learn how you can: Generate uniform 3D cell models, Get the best quality images, Minimize imaging time and volume of data, Get deeper insights from your 3D cell model and Avoid unnecessary data transfer steps.

PDF 1 MB

Technical Note

3D Volumetric Analysis of Luminal Spaces Inside Cysts or Organoids

High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. In this technical note we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS™ high-content analysis system.

PDF 1 MB
How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS™ and Opera Phenix™ high-content systems, avoid phototoxicity with gentle digital phase contrast imaging, and analyze cell growth and morphology on a single cell level without fluorescence staining.

PDF 3 MB
The Benefits of Automated Water Immersion Lenses for High-Content Screening

Balancing the key factors in HCS imaging - sensitivity, resolution and speed - can be challenging since they cannot be optimized independently: changing one impacts the others. Nevertheless, there is a way to overcome some of the obstacles and here we explain why the choice of the objective lens is critical.

PDF 1 MB