The Opera Phenix HCS system's innovative optical design lets you generate richer information through extremely sensitive confocal imaging and at higher throughput than ever through simultaneous acquisition – without the issue of crosstalk.
Speed and Sensitivity – No Compromise
Proprietary Synchrony™ Optics combine a microlens enhanced Nipkow spinning disk with dual view confocal optics to separate fluorescence excitation and emission during simultaneous acquisition minimizing spectral crosstalk – for more speed and more sensitivity.
- Up to four large format sCMOS cameras with simultaneous acquisition in up to four channels, so you can generate images of exceptional quality - faster than ever
- Custom-designed high NA water immersion objectives to capture more photons and provide high image resolution even in thick samples
- Large field of view – 290% larger than the original Opera HCS system to acquire more cells in a single image for robust assay statistics
- State-of-the-art sCMOS image sensors deliver low signal-to-background noise, a wide dynamic range and high resolution – ideal for sensitive and quantitative measurements at short exposure times
- Spinning microlens disk with increased pinhole-to-pinhole distance to further reduce out-of-focus noise in thick samples such as microtissues
- Spinning disk optics and precise synchronization of the excitation laser and camera to minimize phototoxicity and bleaching for live cell assays
- Widefield or confocal imaging modes depending on your assay requirements
- Fast laser-based autofocus to ensure sharp confocal images from every field
- Modular design to adapt the system to your changing application needs – choose from single, dual or four camera configuration with four solid state lasers or a five laser system optimized for FRET applications
With the Opera Phenix HCS System you can generate high resolution images in up to four colors at ultra-high throughput.
It All Comes Together in Harmony – Acquisition to Analysis Made Easy
- Intuitive workflow user-interface guides you through the entire process, from acquisition to analysis and evaluation
- Templates for set-up of acquisition channels and parameters to get you productive right away
- Ready-made solutions for common image analysis tasks so you can simply select and go
- Image analysis building blocks that allow you to create, configure, and customize your own high content analysis applications
- Advanced analysis features such as texture and STAR morphology analysis for detailed descriptions of cellular morphology and robust differentiation of cellular phenotypes
- Data management for automatic storage of analysis results and metadata, such as assay layout, instrument, settings, plus user-defined keywords, and annotations
The Harmony software has an intuitive user-interface that guides you through the whole process from acquisition to analysis and evaluation.
Your Total Solution for High Content Screening
- Get better results from your Opera Phenix system with our for high content screening, such as the CellCarrier™ Ultra 384-well microplates designed for optimal performance in high content imaging applications and our CellCarrier Spheroid ULA plates for imaging 3D models.
- Improve throughput and productivity, and reduce variability and reagent costs by automating your Opera Phenix system, for example with the cell::explorer™ automated workstation
- Export your results automatically into the Columbus™ Image Data Storage and Analysis System, so you can access, reanalyze, store, and share image data from Opera Phenix and other HCS systems across your organization
- NEW! Export your results to High Content Profiler™, powered by TIBCO Spotfire®, and perform screening data analysis and validation, QC analyses, calculate reliable normalization, perform multivariate hit stratification and drug response profiling
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- Li, M; Wang, X; Cao, L; Lin, Z; Wei, M; Fang, M and Li, S. 2016. Quantitative and epitope-specific antigenicity analysis of the human papillomavirus 6 capsid protein in aqueous solution or when adsorbed on particulate adjuvants. Vaccine 34(37) 4422-4428.
- Phillips, SL; Soderblom, EJ; Bradrick, SS; Garcia-Blanco, MA. 2016. Identification of proteins bound to dengue viral RNA in vivo reveals new host proteins important for virus replication. mBio 7(1):e01865-15. doi: 10.1128/mBio.01865-15.
- Whalley, HJ et al. 2015. Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat. Commun.6:7437 doi: 10.1038/ncomms8437
Introducing the New Operetta CLS™ High-Content Analysis System
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