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Opera Phenix High-Content Screening System

The Opera Phenix High Content Screening System is the premier confocal solution for today's most demanding high content applications. Drawing on over a decade of experience with the industry-leading Opera® High Content Screening System, the Opera Phenix is designed for high-throughput, phenotypic screening and assays involving complex disease models, such as live cells, primary cells and microtissues.

Part Number HH14000000
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For research use only. Not for use in diagnostic procedures.

Detail Information

The Opera Phenix HCS system's innovative optical design lets you generate richer information through extremely sensitive confocal imaging and at higher throughput than ever through simultaneous acquisition – without the issue of crosstalk.

Speed and Sensitivity – No Compromise

Proprietary Synchrony™ Optics combine a microlens enhanced Nipkow spinning disk with dual view confocal optics to separate fluorescence excitation and emission during simultaneous acquisition minimizing spectral crosstalk – for more speed and more sensitivity.

  • Up to four large format sCMOS cameras with simultaneous acquisition in up to four channels, so you can generate images of exceptional quality - faster than ever
  • Custom-designed high NA water immersion objectives to capture more photons and provide high image resolution even in thick samples
  • Large field of view – 290% larger than the original Opera HCS system to acquire more cells in a single image for robust assay statistics
  • State-of-the-art sCMOS image sensors deliver low signal-to-background noise, a wide dynamic range and high resolution – ideal for sensitive and quantitative measurements at short exposure times
  • Spinning microlens disk with increased pinhole-to-pinhole distance to further reduce out-of-focus noise in thick samples such as microtissues
  • Spinning disk optics and precise synchronization of the excitation laser and camera to minimize phototoxicity and bleaching for live cell assays
  • Widefield or confocal imaging modes depending on your assay requirements
  • Fast laser-based autofocus to ensure sharp confocal images from every field
  • Modular design to adapt the system to your changing application needs – choose from single, dual or four camera configuration with four solid state lasers or a five laser system optimized for FRET applications

With the Opera Phenix HCS System you can generate high resolution images in up to four colors at ultra-high throughput.

It All Comes Together in Harmony – Acquisition to Analysis Made Easy

  • Intuitive workflow user-interface guides you through the entire process, from acquisition to analysis and evaluation
  • Templates for set-up of acquisition channels and parameters to get you productive right away
  • Ready-made solutions for common image analysis tasks so you can simply select and go
  • Image analysis building blocks that allow you to create, configure, and customize your own high content analysis applications
  • Advanced analysis features such as texture and STAR morphology analysis for detailed descriptions of cellular morphology and robust differentiation of cellular phenotypes
  • Data management for automatic storage of analysis results and metadata, such as assay layout, instrument, settings, plus user-defined keywords, and annotations

The Harmony software has an intuitive user-interface that guides you through the whole process from acquisition to analysis and evaluation.

Your Total Solution for High Content Screening

  • Get better results from your Opera Phenix system with our for high content screening, such as the CellCarrier™ Ultra 384-well microplates designed for optimal performance in high content imaging applications and our CellCarrier Spheroid ULA plates for imaging 3D models.
  • Improve throughput and productivity, and reduce variability and reagent costs by automating your Opera Phenix system, for example with the cell::explorer™ automated workstation
  • Export your results automatically into the Columbus™ Image Data Storage and Analysis System, so you can access, reanalyze, store, and share image data from Opera Phenix and other HCS systems across your organization
  • NEW! Export your results to High Content Profiler™, powered by TIBCO Spotfire®, and perform screening data analysis and validation, QC analyses, calculate reliable normalization, perform multivariate hit stratification and drug response profiling


  • Barrows, NJ; Campos, RK; Powell, ST; Prasanth, KR. 2016. A Screen of FDA-Approved Drugs for Inhibitors of Zika Virus Infection. Cell Host & Microbe, doi:10.1016/j.chom.2016.07.004
  • Conos, SA; Lawlor, KE; Vaux, DL; Vince, JE. 2016. Cell death is not essential for caspase-1-mediated interleukin-1ß activation and secretion. Cell Death & Differentiation, doi:10.1038/cdd.2016.69
  • Fraietta, I. and Gasparri, F. 2016. The development of high-content screening (HCS) technology and its importance to drug discovery. Expert Opinion on Drug Discovery, Vol.11, 5. DOI:10.1517/17460441.2016.1165203
  • Kleensang, A; Vantangoli, MM; Odwin-DaCosta, S. 2016. Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function. Scientific Reports 6, Article number: 28994, doi:10.1038/srep28994
  • Letzsch, S; Boettcher, K; Kelm, JM. and Messner, S. 2015. Quantifying Efflux Activity in 3D Liver Spheroids : New Confocal Imaging Instruments Allow Screening in Complex Human Liver Microtissues. Genetic Engineering & Biotechnology News. 35(7): 14-15. doi:10.1089/gen.35.07.08.
  • Li, L; Zhou, Q; Voss, TC; Quick, KL and LaBarbera, DV. 2016. High-throughput imaging: Focusing in on drug discovery in 3D. Methods 96 (2016) 97–102. doi.10.1016/j.ymeth.2015.11.013
  • Li, M; Wang, X; Cao, L; Lin, Z; Wei, M; Fang, M and Li, S. 2016. Quantitative and epitope-specific antigenicity analysis of the human papillomavirus 6 capsid protein in aqueous solution or when adsorbed on particulate adjuvants. Vaccine 34(37) 4422-4428.
  • Phillips, SL; Soderblom, EJ; Bradrick, SS; Garcia-Blanco, MA. 2016. Identification of proteins bound to dengue viral RNA in vivo reveals new host proteins important for virus replication. mBio 7(1):e01865-15. doi: 10.1128/mBio.01865-15.
  • Whalley, HJ et al. 2015. Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat. Commun.6:7437 doi: 10.1038/ncomms8437

Introducing the New Operetta CLS™ High-Content Analysis System

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21 CFR Part 11 Compatible No
Automation Compatible Yes
Detection Method Transmission, Fluorescence
Imaging Modality High Content
Interface Ethernet
Light Source Laser
Portable No
Product Brand Name Opera Phenix
Warranty Yes
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Application Note

Measuring FRET using the Opera Phenix High-Content Screening System

In this study, we have established a fast and robust high-content FRET-based assay to study protein-protein interactions.



Drug Discovery Screening Solutions Brochure

Info on PerkinElmer's complete solutions for high-throughput and phenotypic screening.

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