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OPAL 6-color fIHC Kit, 250 slides

Opal™ is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.

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Product Number Unit Size List Price Your Price Quantity
NEL796001KT 50 slides 1134.00 USD
NEL796B001KT 250 slides 4957.00 USD

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Opal™ 6-color IHC Kits make multiplex methods accessible to anyone who works with standard immunohistochemistry. The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.

This kit contains 5 reactive fluorophores plus Spectral DAPI and AR6 buffer for multiplex staining protocols. Users should source primary antibodies, HRP conjugates and other workflow reagents separately. The Opal 6-color IHC Kit was configured and validated for imaging on the Vectra and Mantra Systems. Contains enough reagents to stain 250 slides.

Opal™ provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information that is not available from alternative techniques like flow cytometry or analysis of single markers in serial sections. Opal will enable you to:

  • Measure three or more tissue biomarkers at once.
  • Use the best primary antibodies together in multiplex panels, with no species based crosstalk.
  • Retain spatial cellular context that is lost in flow cytometry.
  • Confirm single cell co-expression for many biomarkers in one tissue section.
  • Get more information while conserving precious tissue.



Detection Method Fluorescence
Fluorophore DAPI, Opal 520, Opal 540, Opal 570, Opal 620, Opal 690
Product Brand Name Opal
Quantity in a Package Amount 1.0 per pack
Shipping Condition Blue Ice
Unit Size 250 slides


Resources, Events & More

All (19)
Resource Type File Name File Format
Brochure Phenoptics Brochure PDF 2 MB
Flyer Phenotype and Visualize with Opal Kits PDF 3 MB
Product Info Let's Rid the World of Ribbons - Infographic PDF 822 KB
Opal Multiplex IHC Assay Development Guide Opal Multiplex IHC Assay Development Guide Guide
Phenoptics Research Solutions e-book Phenoptics Research Solutions e-book e-book
Poster Evaluation Of Tumor Immune Infiltrate as a Determinant of Response to Neo-Adjuvant Lapatinib Link
Scientific Paper NEJM: Mutations Associated with Acquired Resistance to PD-1 Blockade in Melanoma Link
Scientific Paper Esbona, Karla, et al. "COX-2 modulates mammary tumor progression in response to collagen density." Breast Cancer Research 18.1 (2016) Link
Scientific Paper Feng, Zipei, et al. "Multispectral Imaging of T and B Cells in Murine Spleen and Tumor." The Journal of Immunology 196.9 (2016): 3943-3950 Link
Scientific Paper Woods, Katherine, et al. "Mismatch in epitope specificities between IFN? inflamed and uninflamed conditions leads to escape from T lymphocyte killing in melanoma." Journal for immunotherapy of cancer 4.1 (2016) Link
Scientific Paper Huang, Fangjin, et al. "Quantitative imaging for development of companion diagnostics to drugs targeting HGF/MET." The Journal of Pathology: Clinical Research (2016) Link
Scientific Paper Ören, Bilge, et al. "Tumour stroma-derived lipocalin-2 promotes breast cancer metastasis." The Journal of Pathology (2016) Link
Scientific Paper Linch, Stefanie N., et al. "Combination OX40 agonism/CTLA-4 blockade with HER2 vaccination reverses T-cell anergy and promotes survival in tumor-bearing mice." Proceedings of the National Academy of Sciences 113.3 (2016): E319-E327 Link
Scientific Paper NEJM: PD-1 Blockade with Pembrolizumab in Advanced Merkel-Cell Carcinoma Link
Scientific Paper Novartis Genoptix Shows Multiplexed IHC Test Better Predicts Immunotherapy Response Link
Scientific Paper Mlecnik, Bernhard, et al. "The tumor microenvironment and Immunoscore are critical determinants of dissemination to distant metastasis." Science translational medicine 8.327 (2016) Link
Story How Your Immune System Can Fight Cancer Link
Webinar Using multiplexed IHC to Effectively Navigate the Hidden Clues of the Immunological Microenvironment Link
Webinar Untangling the tumor microenvironment: Illuminating the complex interactions and functions of immune cells Link