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Opal 540 Reagent Pack

Opal is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.

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Part Number Wave Length Type List Price Your Price Quantity
FP1487001KT 494/525 nm Opal 520 118.00 USD
FP1494001KT 523/536 nm Opal 540 118.00 USD
FP1488001KT 550/570 nm Opal 570 118.00 USD
FP1495001KT 588/616 nm Opal 620 118.00 USD
FP1496001KT 627/650 nm Opal 650 134.00 USD
FP1497001KT 676/694 nm Opal 690 134.00 USD
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Overview

The Opal Reagent Pack contains:

  • Opal Reagent, 1 vial
  • Dimethyl sulfoxide (DMSO), 1 x 100 uL

Users should source primary antibodies and other workflow reagents separately. Before each procedure, dilute reconstituted Opal Fluorophore in 1X Amplification Diluent (not included) to make Opal Fluorophore Working Solution. Pack contains enough reagent to stain 50-slides with the following dilution ratios:

  • 1:100 for manual kits
  • 1:150 for automated kits

Generally, 100-300 µL of Opal Working Solution is required per slide.

The Opal Reagent Packs can be utilized for both mono- and multiplex tissue staining and have been configured and validated for imaging on the Vectra® and Mantra systems.

Note: If you have never used Opal Multiplex IHC Detection Reagents before, we strongly suggest purchasing the full Opal kit, which includes a set of quality controlled reagents that have been optimized to work together.

Opal Multiplex IHC Kits make multiplex methods accessible to anyone who works with standard immunohistochemistry. The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.

Specifications

Shipping Condition Blue Ice
Type Opal 540
Unit Size 1 kit
Wave Length 523/536 nm

Resources, Events & More

Resource Type File Name File Format
Brochure Brochure: Phenoptics Portfolio PDF  2 MB
Data Sheet Data Sheet: Opal 540 Reagent Pack (FP1494001KT) PDF  19 KB
Support Pages Application Support Knowledgebase: Opal Multiplex IHC Assay FAQs Link