Opal™ is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|NEL800001KT||4-color kit||856.00 USD|
|NEL801001KT||7-color kit||1132.00 USD|
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The Opal 4-Color Automation IHC Kit contains:
Users should source primary antibodies and other workflow reagents separately. The Opal 4-Color Automation IHC Kit was optimized for use on the Leica Biosystems BOND RX and configured and validated for imaging on the Vectra® and Mantra™ systems. Kits contain enough reagents to stain 50-slides at the recommended 1:150 dilution ratio.
What is Opal? Opal Multiplex IHC Kits make multiplex methods accessible to researchers who work with standard immunohistochemistry (IHC). The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may now be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.
Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.
Opal will enable you to:
Automating Opal staining brings the benefit of:
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Shipping Condition||Blue Ice|
|Unit Size||4-color kit|
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|Resource Type||File Name||File Format|
|Brochure||Brochure: Phenoptics Portfolio||PDF 2 MB|
|Application Note||Opal Automation Application Note||PDF 1 MB|
|Data Sheet||Data Sheet: Opal 4-7 color Automation IHC Kits||PDF 135 KB|
|Flyer||Flyer: Opal Multiplex Automation IHC Kits||PDF 1 MB|
|Flyer||Flyer: Opal Multiplex IHC Detection Kits||PDF 3 MB|
|E-book||Download Phenoptics Research Solutions E-book||Link|
|Guide||Guide: Opal Assay Development||Link|
|Scientific Poster||Poster: Optimization Strategy For Fluorescence Multiplex Immunohistochemistry Tissue Staining||Link|
|Scientific Poster||Poster: Automate Your Complete Cancer Research Workflow with Phenoptics™||Link|
|Support Pages||Application Support Knowledgebase: Opal Multiplex IHC Assay FAQs||Link|
|Webinars||Webinar: Untangling the tumor microenvironment: Illuminating the complex interactions and functions of immune cells||Link|
|Webinars||Webcast: The Beginning of the End - Immunoprofiling Coming of Age||Link|
|Webinars||Webinar: Using multiplexed IHC to Effectively Navigate the Hidden Clues of the Immunological Microenvironment||Link|