PerkinElmer
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Opal 4-Color Automation IHC Kit 50 slides

Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.

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Part Number Unit Size List Price Your Price Quantity
NEL800001KT 4-color kit 856.00 USD
NEL801001KT 7-color kit 1132.00 USD
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Overview

The Opal 4-Color Automation IHC Kit contains:

  • 3 reactive fluorophores (Opal 520, Opal 570 and Opal 690)
  • Spectral DAPI
  • 1 X Plus Amplification Diluent
  • Blocking/Ab Diluent
  • Opal Polymer HRP Ms + Rb

Users should source primary antibodies and other workflow reagents separately. The Opal 4-Color Automation IHC Kit was optimized for use on the Leica Biosystems BOND RX and configured and validated for imaging on the Vectra® and Mantra systems. Kits contain enough reagents to stain 50-slides at the recommended 1:150 dilution ratio.

What is Opal? Opal Multiplex IHC Kits make multiplex methods accessible to researchers who work with standard immunohistochemistry (IHC). The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may now be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.

Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.

Opal will enable you to:

  • Measure up to six tissue biomarkers at once
  • Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
  • Retain spatial cellular context that is lost in flow cytometry
  • Confirm single cell co-expression for many biomarkers in one tissue section
  • Get more information while conserving precious tissue.

Automating Opal staining brings the benefit of:

  • Quality, consistency and reproducibility with every sample
  • Time-savings versus laborious manual process
  • Ease-of-use with a walk-away, high-throughput protocol

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Shipping Condition Blue Ice
Unit Size 4-color kit