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Nicotine, L-(-)-[N-Methyl-3H]-, 250µCi (9.25MBq)

Nicotine radioligand tritiated on the methyl residue. Nicotine acts as an agonist at most nicotinic acetylcholine receptors; however it acts as an antagonist at two nicotinic receptor subunits (nAChRα9 and (nAChRα10). 

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25 µCi
499.00 USD
250 µCi
1656.00 USD
1 mCi
3850.00 USD
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Detail Information

Nicotine functions as an agonist at most nicotinic acetylcholine receptors, except at two nicotine receptor subunits (nAChRα9 and (nAChRα10), where it functions as an antagonist. While nicotine is well known to be a stimulant, its effects actually change from stimulate to sedative with increasing dosages. There is evidence that nicotine provides a potential benefit in both Alzheimer’s and Parkinson’s diseases.

We offer both agonist and antagonist radioligands for autoradiographic visualization or performing saturation and competition assays to determine receptor expression levels (Bmax), dissociation constants (Kd), association and dissociation rates (kon and koff), and inhibitor constants (Ki) for a variety of receptors. We develop our radioligands to the highest specific activity possible and provide high purity resulting in low non-specific binding products. Each of our ultra-pure radioligands is fully characterized for pharmacological action and validated in receptor binding assays.

Radiolabeled ligands remain the most sensitive method for probing receptor binding biology; yielding unprecedented sensitivity gives results that technicians and scientists can trust. For over 50 years, PerkinElmer has been a leading supplier of New England Nuclear (NEN) radiochemicals, liquid scintillation cocktails, vials and nuclear counting detection instruments.

References for NET827:

  1. Amelia Marutle, Per-Göran Gillberg, Assar Bergfors, Wenfeng Yu, Ruiqing Ni, Inger Nennesmo, Larysa Voytenko and Agneta Nordberg. 33H-PIB autoradiography show different laminar distributions of astroglia and fibrillary β-amyloid in Alzheimer brain. Journal of Neuroinflammation. 2013.
  2. Manolo Mugnaini, Michela Tessari, Giorgio Tarter, Emilio Merlo Pich, Cristiano Chiamulera, Bernd Bunnemann. Upregulations of [3H]metyllycaconitine binding sites following continuous infusion of nicotine, without changes of α7 or α6 subunit mRNA: an autoradiography and in situ hybridization study in rat brain. European Journal of Neuroscience. November 3003. Volume 16, Issue 9, pages 1633-1646.
  3. Elisabet Jerlhag, Emil Egecioglu, Suzanne L. Dickson, Lennart Svensson, Jörgen A. Engela. Alpha-conotoxin MII-sensitive nicotine acetylcholine receptors are involved in mediating the ghrelin-induced locomotor stimulation and dopamine overflow in nucleus accumbens. European Neuropsychopharmacology. July 2008. Volume 18, Issue 7, pages 508-518.


Detection Method Radiometric
Label Position Specifically Labeled
Molecular Weight 162.2
Product Brand Name NEN Radiochemicals
Radioisotope H-3
Radionuclide 3H
Receptor Type Nicotinic
Shipping Condition Dry Ice
Special Ordering Information This is a radioactive product - shipping address must have a license to receive radioactive materials.
Therapeutic Area CNS & Neurologic, Pain, Inflammation
Unit Size 250 µCi