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AlphaScreen Mouse IgG Detection Kit, 50,000 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-mouse IgG conjugated AlphaScreen Acceptor beads.

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NULL OR EMPTY CART

Product Number Unit Size List Price Your Price Quantity
6760606C 500 assay points 170.00 USD
6760606M 10,000 assay points 2712.00 USD
6760606R 50,000 assay points 13000.00 USD

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Overview

This bead kit can be used for many applications, including:

  • Antibody detection assays

  • Protein detection assays

  • Protein-protein interaction assays

  • Protein expression

  • Binding studies

    In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is an anti-species antibody, and associates with the corresponding AlphaScreen anti-species Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated FITC probe provided in the kit.  This kit contains enough reagents to run 50,000 wells in 384-well format, using a 25 µL reaction volume.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugates Streptavidin/Anti-mouse IgG
Automation Compatible Yes
Product Brand Name AlphaScreen
Shipping Condition Blue Ice
Unit Size 50,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha Technology Solutions PDF 4 MB