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IgE (mouse) isotyping AlphaLISA Detection Kit, 500 Assay Points

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgE antibodies in different sample matrices, including cell culture media. The IgE subtype plays a role in allergic reactions, parasitic infections and hypersensitivity reactions.

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Product Number Unit Size List Price Your Price Quantity
AL526HV 1 Kit 584.00 USD
AL526C 500 assay points 1378.00 USD
AL526F 5,000 assay points 9100.00 USD

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  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. IgE has two main immune functions, it is the main driver in the immune resonse to parasites and it also is the main cause of allergic reactions. IgE is typically the least abundant immunoglobulin in serum but is capable of triggering the most powerful inflammation reations of all the isotypes. Typically mouse serum and plasma samples contain less than 75 ng/mL of IgE.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target IgE
Assay Target Class Antibody
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 500 data points
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Biologics/Bioprocess
Unit Size 500 assay points

Resource Library & Events

Resource Type File Name File Format
Data Sheet Manual AlphaLISA Mouse IgE Isotyping AL526 PDF 274 KB
Poster AlphaLISA Isotyping Automation Poster PDF 767 KB