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IgA (mouse) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

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Product Number List Price Your Price Quantity
AL528HV 584.00 USD

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  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. IgG is involved in response to a foreign antigen. IgA is the main Ig in mucosal tissue and is believed to be involved in defense against viral and bacterial infections in these locations. Typically mouse serum and plasma samples contain about 0.075 to 0.45 mg/ml of IgA depending on the age and strain of mice. The present kit permits detection of mouse IgA (i.e. analyte) in different sample matrices, including different cell culture media.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target IgA
Assay Target Class Antibody
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 1 kit
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Biologics/Bioprocess
Unit Size 100 assay points

Resource Library & Events

Resource Type File Name File Format
Data Sheet Manual AlphaLISA Mouse IgA Isotyping AL528 PDF 277 KB