PerkinElmer
check quantity

TSH (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Part Number Unit Size List Price Your Price Quantity
TRF3005C 500 Assay Points 773.00 USD
TRF3005M 10,000 Assay Points 10300.00 USD
Buy Now

You successfully added item(s) to your cart

Overview

The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

TSH (thyroid stimulating hormone), also known as thyrotropin, is a member of the cysteine knot growth factor superfamily. It is a heterodimer consisting of a 15 kDa unique TSH beta subunit and a 14 kDa alpha subunit, common glycoprotein hormone alpha that is shared with Luteinizing Hormone, Follicle Stimulating Hormone and Human Chorionic Gonadotropin. Production of TSH by the anterior pituitary gland is stimulated by the hypothalamic peptide TRH. TSH binds to thyroid TSH receptors to stimulate production of thyroxine (T4). In tissues, T4 is converted to the active form of thyroid hormone, triiodothyronine (T3), which stimulates the metabolism of almost every tissue in the body and completes a feedback loop by inhibiting TSH production. Serum TSH measurement is a crucial tool for the diagnosis of thyroid disorders. Increased serum TSH is an early and sensitive indicator of decreased thyroid reserve and overt primary hypothyroidism. Decreased TSH level is an indicator of TSH-independent hyperthyroidism (Graves' disease). For recombinant hTSH, conversion for IU is 1 µg of protein equals 7.9 x 10-3 IU.

Specifications

Assay Target TSH
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra TSH (Human) Detection Kit TRF3005 C/M Manual PDF  557 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB