The LANCE® Ultra Human MANF Detection Kit is designed for detection and quantitation of human MANF in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
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The LANCE® Ultra Human MANF Detection Kit is designed for detection and quantitation of human MANF (ARMET) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a 20 kDa protein systhesized and localized in the endoplasmic reticulum and golgi apparatus of several cell types. The protein is also known as arginine rich, mutated in early stage tumors (ARMET) and arginine rich protein (ARP) and is secreted into the peripheral tissues and organs. This protein is associated with several pathologies such as Parkinson’s disease, Type 1 Diabetes, and tumors.
|Assay Target Class||Protein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
This manual explains how to run the LANCE Ultra TR-FRET human MMP-7 detection assay.