PerkinElmer
check quantity

C-peptide (mouse/rat) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Mouse C-peptide Detection Kit is designed for detection and quantitation of Mouse C-peptide in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Part Number Unit Size List Price Your Price Quantity
TRF1510C 500 Assay Points 773.00 USD
TRF1510M 10,000 Assay Points 10300.00 USD
Buy Now

You successfully added item(s) to your cart

Overview

The LANCE® Ultra Mouse C-peptide Detection Kit is designed for detection and quantitation of Mouse C-peptide in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

 

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

 

Mature Connecting Peptide (C-Peptide) is a 31 amino acid peptide that is a product from the proteolytic processing of proinsulin. In mouse and rat, contrary to humans, two isoforms of C-Peptide are present (isoforms 1 and 2). Through its G-protein coupled membrane receptor, C-Peptide activates Ca2+-dependent intracellular signaling pathways, resulting in subsequent activation of both Na+ /K+ ATPase and endothelial nitric oxide synthase. C-Peptide and insulin are co-secreted in equimolar amounts in the bloodstream by pancreatic beta cells of the islets of Langerhans. However, C-Peptide has a longer half-life than insulin in serum, making it a good indicator of endogenous insulin production. A lack of endogenous C-Peptide contributes to development of long-term microvascular complications in the nerves, the kidneys, and the retina in Type 1 diabetes patients. C-Peptide has been considered as a possible therapy to prevent long-term complications of diabetes.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target C-peptide
Assay Target Class Peptide
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Data Sheet Manual: LANCE Ultra Mouse/Rat C-Peptide Kit PDF  468 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB