PerkinElmer
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Kappa Light Chain (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human Kappa Light Chain Detection Kit is designed for detection and quantitation of human kappa light chain in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF3023C
500 Assay Points
892.00 USD
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TRF3023M
10,000 Assay Points
11900.00 USD
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Detail Information

The LANCE® Ultra Human Kappa Light Chain Detection Kit is designed for detection and quantitation of human kappa light chain in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Most mammal IgG class antibodies, including those from human are composed of two heavy and two light chains. Such light chains can be of kappa or lambda types, with an expected ratio of 3 kappas to one lambda. Both appear to play the same function in antibodies. Free light chains are usually rare in blood. However, several types of multiple myelomas will secrete free light chains, which can then accumulate in organs such as the kidney and cause severe damage, with lambda chains being the poorest diagnostic. Knowledge of the type of light chain generated is also important for the creation and production of therapeutic antibodies. Techniques used for purification, such as protein L or anti species antibodies, can have different affinities for the two chains.

Specifications

Assay Target Kappa light chain
Assay Target Class Antibody
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Biologics/Bioprocess
Unit Size 10,000 Assay Points