PerkinElmer
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IL-28B (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human IL-28B Detection Kit is designed for detection and quantitation of human IL-28B in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1365C 500 Assay Points 866.00 USD
TRF1365M 10,000 Assay Points 11500.00 USD
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Overview

The LANCE® Ultra Human IL-28B Detection Kit is designed for detection and quantitation of human IL-28B in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Interleukin 28B (IL28B), recently renamed interferon-λ 3 (IFNL3), is a cytokine encoded by the IL28 gene and is closely related to IL18A and IL29. IL28B plays a critical role in adaptive immune responses and regulates CD8+ T cell functions during vaccination. Clinically, it is involved in the protection from H1N1 viral infection and it can be used for predicting response to hepatitis C treatment with interferon and ribavirin. The present kit is designed to detect IL28B in human serum, plasma, and cell culture supernatants.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL28B
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra Human IL-28B Detection kit TRF1365 PDF  478 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB