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IL-12/p40 (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human IL-12/p40 Detection Kit is designed for detection and quantitation of human interleukin-12/p40 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1382C 500 Assay Points 773.00 USD
TRF1382M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra Human IL-12/p40 Detection Kit is designed for detection and quantitation of human IL-12 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Interleukin 12 p40 (IL12 p40) (also known as IL-12B, natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) is a subunit of interleukin 12. It also serves as a subunit of Interleukin 23. This cytokine is involved in atopic diseases, asthma, eczema, rhinitis, and autoimmune diseases. IL12 appears to play a major role in auto-immune disease, in the resistance to bacterial and parasitic infections, in antiviral responses, and in the promotion of antitumor immunity. Increased plasma levels of IL12 p40 have been reported in certain neurological disorders and autoimmune diseases.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL12
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual Manual: LANCE Ultra Human IL-12 Detection kit TRF1382 PDF  493 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB