PerkinElmer
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IgE (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human IgE Detection Kit is designed for detection and quantitation of human immunoglobulin E in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1292C 500 Assay Points 750.00 USD
TRF1292M 10,000 Assay Points 10000.00 USD
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Overview

The LANCE® Ultra Human IgE Detection Kit is designed for detection and quantitation of human IgE in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Immunoglobulin E (IgE) is a monomeric antibody with four Ig-like domains. Compared to all other immunoglobulin classes, IgE is typically the least abundant isotype in plasma of normal healthy individuals. IgE binds with extremely high affinity to Fc-epsilon receptor I (FcɛRI), which is expressed on mast cells, basophils, Langerhans cells, and eosinophils. Circulating IgE upregulates FcɛRI expression on these cells. The combination of strong binding and upregulation of FcɛRI expression contributes to the remarkable potency of this immunoglobulin. IgE is associated with hypersensitivity and allergic reactions, as well as the response to parasitic worm infections.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IgE
Assay Target Class Antibody
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Biologics/Bioprocess
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra Human IgE Detection Kit TRF1292 PDF  516 KB