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Transferrin (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human Transferrin Detection Kit is designed for detection and quantitation of human transferrin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1311C 500 Assay Points 773.00 USD
TRF1311M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra Human Transferrin Detection Kit is designed for detection and quantitation of human transferrin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Human Transferrin is a 679 amino acid protein (80 kDa) composed of alpha helices and beta sheets that form two distinct domains that each contain an iron binding site. This protein functions to control the level of free iron found in biological fluids. The liver is the main site of transferrin synthesis and its main role is to deliver iron to all tissues. Normal human serum transferrin levels range from 200-360 mg/dL. The transferrin protein has been implicated in several disease states including iron deficiency anemia (high serum transferrin levels) and liver toxicity. The present kit permits detection of human transferrin (i.e. analyte) in different sample matrices, including DMEM cell culture media.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Transferrin
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra Human Transferrin TRF1311 PDF  498 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB