PerkinElmer
check quantity

Tau (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human TAU Detection Kit is designed for detection and quantitation of human Tau in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Part Number Unit Size List Price Your Price Quantity
TRF1271C 500 Assay Points 772.00 USD
TRF1271M 10,000 Assay Points 10300.00 USD
Buy Now

You successfully added item(s) to your cart

Overview

The LANCE® Ultra Human TAU Detection Kit is designed for detection and quantitation of human tau in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Microtubule-associated Protein Tau (TAU) is a neuronal protein that binds to axonal microtubules and has roles in the assembly of microtubules, cytoskeletal structure, and axonal transport. Six isoforms of Tau have been described, ranging from 352 to 441 residues, all originating from the alternative splicing of one gene designated MAPT. Tau interacts with tubulin to stabilize the microtubules, but its phosphorylation blocks this association, resulting in disruption of microtubule organization. In humans, hyperphosphorylated Tau can self-assemble into very insoluble aggregates (paired helical filaments) leading to various pathologies. Tauopathy is the name given to diseases associated with Tau accumulation, among which Alzheimer’s disease (AD) is one of the best known. Tau is a biochemical marker for this neurodegenerative disease as an increase of Tau in cerebrospinal fluid is observed in most patients with AD.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Tau
Assay Target Class Peptide
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Central Nervous System
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra Human Tau Detection Kit TRF1271 PDF  485 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB