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Perlecan (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human Perlecan Detection Kit is designed for detection and quantitation of human perlecan in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1377C 500 Assay Points 773.00 USD
TRF1377M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra Human Perlecan Detection Kit is designed for detection and quantitation of human perlecan in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Perlecan is a large (4391 AA) multidomain protein, one of them being Endorepellin (3687 – 4391 AA), synthesized primarily by vascular endothelial cells and deposited in the vascular extracellular matrix. Its function is to crosslink a variety of matrix components to maintain the endothelial barrier function. Further, it can stimulate growth and regeneration of the endothelium via the modulation of certain growth factor activities. Perlecan has garnered significant interest due to its ability to suppress tumor growth upon its own suppression. Also, its levels have been observed to decrease in other diseases like atherosclerosis and diabetes.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Perlecan
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Unit Size 500 Assay Points