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Insulin (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1204C 500 Assay Points 773.00 USD
TRF1204M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Insulin is synthesized as a proinsulin hormone of 110 aa by Beta-cells of the islets of Langherans in the pancreas. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A of 21 aa and peptide B of 30 aa) that are covalently linked via two disulfide bonds and secreted upon increased glucose concentration in blood. Blood concentration increases from around 50 pmol/L to 300-400 pmol/L 30 min after glucose uptake. Insulin is a key player in the control of both carbohydrate and lipid metabolism and has been implicated in various diseases including diabetes, heart disease and obesity.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Insulin
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra Human Insulin TR-FRET Detection Kit PDF  553 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB