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Insulin (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF1204C
500 Assay Points
796.00 USD
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TRF1204M
10,000 Assay Points
10600.00 USD
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Detail Information

The LANCE® Ultra Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Insulin is synthesized as a proinsulin hormone of 110 aa by Beta-cells of the islets of Langherans in the pancreas. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A of 21 aa and peptide B of 30 aa) that are covalently linked via two disulfide bonds and secreted upon increased glucose concentration in blood. Blood concentration increases from around 50 pmol/L to 300-400 pmol/L 30 min after glucose uptake. Insulin is a key player in the control of both carbohydrate and lipid metabolism and has been implicated in various diseases including diabetes, heart disease and obesity.

Specifications

Assay Target Insulin
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 10,000 Assay Points