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IgG (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human IgG Detection Kit is designed for detection and quantitation of human IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1205C 500 Assay Points 773.00 USD
TRF1205M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra Human IgG Detection Kit is designed for detection and quantitation of human IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

The glycoprotein immunoglobulin G (IgG), a major effector molecule of the humoral immune response in human, accounts for about 75% of the total immunoglobulins in plasma of healthy individuals whereas IgM, IgA, IgD and IgE, each of which has characteristic properties and functions, constitute the remaining 25%. The basic IgG molecule has a four-chain structure, comprising two identical heavy (H) chains and two identical light (L) chains, linked together by interchain disulfide bonds. Four IgG subclasses have been identified showing their most conspicuous differences in the amino acid composition and structure of the 'hinge region'. The present kit recognizes total IgGs Fc fragment.

 

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IgG
Assay Target Class Antibody
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Biologics/Bioprocess
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual Human IgG LANCE Ultra Detection Kit TRF1205 PDF  463 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB