The LANCE® Ultra Human IFN-α Detection Kit is designed for detection and quantitation of human interferon α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF1297C||500 Assay Points||796.00 USD|
|TRF1297M||10,000 Assay Points||10600.00 USD|
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The LANCE Ultra Human IFN-α Detection Kit is designed for detection and quantitation of human interferon α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Interferon-alpha (IFN-α) is a cytokine involved in innate immune response against viral infection. It is produced by leukocytes and other cell types like plasmacytoid dendritic cells. There are many IFN-α subtypes, with molecular weights ranging from 15 to 21 kDa. They all possess the same antiviral activity, but may differ in their relative biological activities. IFN-α also exhibits several antiproliferative and antitumor activities, making it one of the most used cytokine treatments in cancer patients suffering from hairy cell leukemia, renal carcinoma, Kaposi’s sarcoma, and other malignancies. Under physiological conditions, a low level of IFN-α is detected. However, its production is markedly enhanced during infections and various pathological situations, making IFN-α a major disease marker.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
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|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 82 KB|
|Application Note||High Throughput Quantitation of Cytokine Biomarkers using LANCE Ultra TR-FRET Assays||PDF 1 MB|
|Application Note||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|
|Technical Note||Comparison of LANCE Ultra and HTRF Assays for Measuring the Presence of Pro-inflammatory Cytokines||PDF 277 KB|
|Data Sheet||Tech data sheet: LANCE human IFNa TRF1297||PDF 607 KB|
|Flyer||LANCE Ultra Biomarker Detection Kits||PDF 585 KB|