PerkinElmer
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Glucagon (human) LANCE Ultra TR-FRET Detection Kit, 5,000 Assay Points

The LANCE® Ultra Human Glucagon Detection Kit is designed for detection and quantitation of human glucagon in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1312C 500 Assay Points 772.00 USD
TRF1312M 10,000 Assay Points 10300.00 USD
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Overview

Attention: Glucagon Analyte included in this kit is provided in solution. Please store at -20°C upon receipt of the kit. Upon the first use of the kit aliquot the analyte and store the aliquots at -20°C to avoid further freeze/thaw cycles.

The LANCE® Ultra Human Glucagon Detection Kit is designed for detection and quantitation of human glucagon in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Glucagon is a 29 amino acid peptide hormone produced by the pancreas. Glucagon generally functions as a counterregulatory homrone opposing the actions of insulin to maintain appropriate levels of blood glucose. Normal human serum glucagon levels range from 50-200 pg/mL. The glucagon:insulin ratio controls the rate of gluconeogenesis and glycogenolysis, disruption of this ratio can have severe metabolic implications. The present kit permits detection of glucagon (i.e. analyte) in different sample matrices.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Glucagon
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra Human Glucagon Detection Kit TRF1312 PDF  552 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB