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ErbB2 (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human ErbB2 (HER2) Detection Kit is designed for detection and quantitation of human ErbB2 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1235C 500 Assay Points 773.00 USD
TRF1235M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra Human ErbB2 (HER2) Detection Kit is designed for detection and quantitation of human ErbB2 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Human epidermal growth factor receptor 2 (ERBB2, NEU, or HER2) is a type I membrane glycoprotein that is a member of the ERBB family of tyrosine kinase receptors. It serves as a receptor for the epidermal growth factor (EGF) family of growth factors. HER2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. It is an orphan receptor with no known ligand, as it acts as a heterodimer with other members of the EGF receptors family. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. HER2 can be shed from the cell surface as a soluble protein (sHER2) via proteolytic cleavage by an unidentified protease. sHER2 seems to be an important serum biomarker in certain types of cancer.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target ErbB2
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note High Throughput Quantitation of Cytokine Biomarkers using LANCE Ultra TR-FRET Assays PDF  1 MB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra Human HER2 TRF1235 PDF  494 KB