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CRP (human) LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE® Human CRP Detection Kit is designed for detection and quantitation of human C-reactive protein in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1233C 500 assay points 773.00 USD
TRF1233M 10,000 assay points 10300.00 USD
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Overview

The LANCE Human CRP Detection Kit is designed for detection and quantitation of human C-reactive protein in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

C-reactive Protein (CRP) is an acute-phase serum protein (206 amino acids), present in homopentameric form, that plays a role in the first line of host defense. In response to infection, inflammation, or tissue damage, IL6, IL1, and glucocorticoids induce the production of CRP in the liver. CRP exhibits Ca2+ - dependent binding to ligands such as phosphorylcholine, a constituent of many bacterial and fungal cell walls, or the membrane of injured cells. It also binds to nuclear components of necrotic and apoptotic cells. It has been shown that high levels of CRP in humans are associated with an increased risk of cardiovascular diseases. Its mechanism is not yet completely understood.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target CRP
Assay Target Class Protein
Automation Compatible Yes
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 10,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Technical Note Comparison of LANCE Ultra and HTRF Assays for Measuring the Presence of Pro-inflammatory Cytokines PDF  277 KB
Data Sheet LANCE Ultra hCRP Detection Kit PDF  453 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB