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Adiponectin (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human Adiponectin Detection Kit is designed for detection and quantitation of human adiponectin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1209C 500 Assay Points 773.00 USD
TRF1209M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra Human Adiponectin Detection Kit is designed for detection and quantitation of human adiponectin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Adiponectin (Acrp30, in mouse) is a hormone secreted by differentiated adipocytes that regulates energy homeostasis and glucose and lipid metabolism. It is expressed as a ~35 kDa protein. It has been demonstrated that Adiponectin / Acrp30 plays a major role in controlling whole-body metabolism, particularly by enhancing insulin sensitivity in muscle and liver, and by augmenting the oxydation of fatty acids in muscle.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Adiponectin
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note High Throughput Quantitation of Cytokine Biomarkers using LANCE Ultra TR-FRET Assays PDF  1 MB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra human adiponectin kit TRF1209 PDF  491 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB