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Phospho-eIF2A (Ser51) LANCE Ultra TR-FRET Cellular Detection Kit, 10,000 Assay Points

LANCE Ultra phospho-eIF2A (Ser51) kits are designed for the detection of eIF2A (phosphorylated at S51) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

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Part Number Unit Size List Price Your Price Quantity
TRF4008C 500 Assay Points 1450.00 USD
TRF4008M 10,000 Assay Points 6500.00 USD
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Overview

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4008S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

Eukaryotic initiation factor 2 (eIF2) is a heterotrimer which is involved in the initiation of translation in eukaryotes. EIF2 binds to two other partners, GTP and Met-tRNAi, and forms the 43S pre-initiation complex upon binding the 40S subunit. Under conditions of cellular stress, the alpha subunit can be phosphorylated at S51 to downregulate protein translation.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target eIF2α
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Shipping Condition Blue Ice
Target Species Human
Unit Size 10,000 Assay Points