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EGFR (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1340C 500 Assay Points 773.00 USD
TRF1340M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra Human EGFR Detection Kit is designed for detection and quantitation of human EGFR in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Epidermal Growth Factor Receptor (EGFR, Her1, ErbB1) is a 134 kDa cell surface receptor apart of a four member subfamily of receptor tyrosine kinases (erbB1, erbB2, erbB3, and erbB4). EGFR binds to a family of proteins called the epidermal growth factors, which upon association induces dimerization of the receptor and initiates signal transduction for the promotion of cell survival and growth. Mutations that cause EGFR overexpression have been linked with many different types of cancer such as lung cancer or gastrointestinal tract cancers. Measuring the EGFR levels is vital for the detection of these types of cancers and is also an important target for therapeutic intervention.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target EGFR
Assay Target Class Receptor
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra EGFR Detection Kit TRF1340 PDF  514 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB