PerkinElmer
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EGF-EGFR LANCE Ultra TR-FRET Binding Kit, 10,000 Assay Points

This kit is designed for the detection of binding activity between Human EGF and EGFR using a homogeneous LANCE Ultra TR-FRET assay. This assay can facilitate the design and development of therapeutics which competitively inhibit EGF/EGFR interaction.

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Part Number Unit Size List Price Your Price Quantity
TRF1366C 500 Assay Points 750.00 USD
TRF1366M 10,000 Assay Points 10000.00 USD
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Overview

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. Streptavidin is labeled with a donor fluorophore (a LANCE Europium chelate) which binds to biotinylated EGF and the EGFR-Fc is recognized by anti-human IgG labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Epidermal Growth Factor Receptor (EGFR, Her1, ErbB1) is a 134 kDa cell surface receptor, part of a four member subfamily of receptor tyrosine kinases (erbB1, erbB2, erbB3, and erbB4). EGFR binds to a family of proteins called the epidermal growth factors (primarily EGF), which, upon association induces dimerization of the receptor and initiates signal transduction for the promotion of cell survival and growth. Mutations that cause aberrant overexpression of EGF and/or EGFR have been linked with many different cancers such as non-small-cell lung and colon cancers. Drugs are being developed as a means to better treat these types cancer.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target EGF,EGFR
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Manual LANCE Ultra EGF-EGFR Binding Kit TRF1366 PDF  517 KB