PerkinElmer
check quantity

CHO-P LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE® Ultra CHO-P Detection Kit is designed for detection and quantitation of CHO proteins in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Part Number Unit Size List Price Your Price Quantity
TRF1210C 500 assay points 773.00 USD
TRF1210M 10,000 assay points 10300.00 USD
Buy Now

You successfully added item(s) to your cart

Overview

The LANCE Ultra CHO-P Detection Kit is designed for detection and quantitation of CHO proteins in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

CHO (Chinese Hamster Ovary) cells are commonly utilized as an expression system for the production of human recombinant proteins at industrial scale. However, since very frequently these proteins are intended to be used as therapeuticals for humans, they must be subsequently purified to remove undesired contaminants that could elicit toxic or immunological reactions. Therefore, there is a current need to quantitate and trace these unwanted host cell proteins in protein preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target CHO-P
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species CHO
Therapeutic Area Biologics/Bioprocess
Unit Size 10,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Technical Note Comparison of LANCE Ultra and HTRF Assays for Measuring the Presence of Pro-inflammatory Cytokines PDF  277 KB
Data Sheet LANCE Ultra CHO-P Detection Kit TRF1210 PDF  452 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB