The LANCE® Ultra Human CCL5/RANTES Detection Kit is designed for detection and quantitation of human CCL5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF1289C||500 Assay Points||796.00 USD|
|TRF1289M||10,000 Assay Points||10600.00 USD|
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The LANCE Ultra Human CCL5/RANTES Detection Kit is designed for detection and quantitation of human CCL5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
C-C Motif Chemokine 5 (CCL5), also known as Regulated upon Activation, Normal T cell Expressed and Secreted (RANTES), is a 7.8 kDa protein that plays a primary role in the inflammatory immune response. CCL5 binds to C-C chemokine receptor (CCR) types 1, 3, and 5. CCL5 dimerization and oligomerization, as well as binding to glycosaminoglycan, are key elements of cell recruitment in the inflammation process. CCL5 is a potent chemoattractant for a number of different cell types, particularly eosinophils, basophils, and mononuclear cells. CCL5 is a potential biomarker for several diseases, for example, it can be useful for indicating the magnitude of asthmatic airway inflammation. In addition to being an important biomarker, CCL5 is studied for its interaction with its receptor, CCR5, in human immunodeficiency virus (HIV) research.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
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|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 77 KB|
|Application Note||High Throughput Quantitation of Cytokine Biomarkers using LANCE Ultra TR-FRET Assays||PDF 1 MB|
|Application Note||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|
|Technical Note||Comparison of LANCE Ultra and HTRF Assays for Measuring the Presence of Pro-inflammatory Cytokines||PDF 277 KB|
|Data Sheet||LANCE Ultra Human CCL5 Detection Kit TRF1289||PDF 463 KB|
|Flyer||LANCE Ultra Biomarker Detection Kits||PDF 585 KB|