The LANCE® Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF1111C||500 Assay Points||773.00 USD|
|TRF1111M||10,000 Assay Points||10300.00 USD|
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The LANCE Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with an acceptor fluorophore [ULight™ dye]. A “tracer” analyte is biotinylated and binds Streptavidin labeled with donor Eu-chelate. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. In the presence of sufficient non-biotinylated analyte this emission will be diminished.
The c-myc polypeptide tag is derived from the c-myc gene. The tag can be used during affinity chromatography and to separate wild-type protein from recombinant protein. The c-myc tag can be used in indirect detection assays for which there is no antibody for the target.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Peptide|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
|Resource Type||File Name||File Format|
|Application Note||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|
|Manual||LANCE Ultra c-Myc Detection kit TRF1111||PDF 632 KB|