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BDNF (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human BDNF Detection Kit is designed for detection and quantitation of human BDNF in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1317C 500 Assay Points 750.00 USD
TRF1317M 10,000 Assay Points 10000.00 USD
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Overview

The LANCE® Ultra Human BDNF Detection Kit is designed for detection and quantitation of human Brain Derived Neurotrophic Factor (BDNF) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Brain Derived Neurotrophic Factor (BDNF) is a neurotrophin that acts within the central and peripheral nervous systems. BDNF is expressed in a pro-form as pro-BDNF then later cleaved by plasmin, furin, or pro-convertase enzymes to become the mature form of BDNF. Released BDNF has been observed to bind to both TrkB and LNGFR (p75) receptors. Acting on these receptors, BDNF helps to support the differentiation of new neurons and synapses, promote neuron growth, and generally promote the survival of existing neurons. BDNF has been observed to potentially play a role in several major diseases such as Alzheimer’s, Parkinson’s and Huntington’s disease.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target BDNF
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Central Nervous System
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra BDNF Detection kit TRF1317 PDF  468 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB