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LANCE Eu-W1024-ITC Chelate and Eu Standard, 100µg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.

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AD0096 2458.00 USD

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Overview

LANCE® Eu-W1024-ITC Chelate is optimized for the labeling of proteins and peptides at least one primary aliphatic amino group. The labeled compound can be used in homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assays (LANCE® assays).

  • Isothiocyanate (ITC) activated chelates react primarily with free amine groups such as ones a N-terminus of protein or lysine residues. 
  • LANCE® W1024 Series chelates are preferred in most HTS applications due to their lower fluorescent background in TR-FRET assays. 
  • For most LANCE™ applications the W1024 series of Eu chelates with active group can be used. Usable pH range 5-11. Under neutral conditions W1024 remains stable in EDTA concentrations up to 20 mmol/L. Allophycocyanin (APC)- or ULight-labeled compounds can be used as acceptors for LANCE® TR-FRET assays.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC)]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024-ITC
Product Brand Name LANCE
Shipping Condition Blue Ice
Unit Size 100 µg

Resources, Events & More

Resource Type File Name File Format
Manual LANCE Eu-W1024 ITC Chelate & Europium Standard AD0096 Development Grade PDF 83 KB