Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in LANCE® TR-FRET kinase assays.
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|Product Number||Unit Size||List Price||Your Price||Quantity|
|AD0066||50 µg||1013.00 USD|
|AD0067||1 mg||8400.00 USD|
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The PY20 antibody (IgG2b) and PT66 antibody are mouse monoclonal antibodies that bind to phosphorylated tyrosine residues. Eu-W1024 labeled anti-phosphotyrosine PY20 antibody for LANCE® assays where the analyte concentration is < 1-10 nM and assay pH > 7. The PY20 antibody is a mouse monoclonal antibody, IgG2b, that binds to phosphorylated tyrosine residues.
Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|Detection Method||TR-FRET, Time-Resolved Fluorescence (TRF)|
|Experimental Type||In vitro|
|Product Brand Name||LANCE|
|Shipping Condition||Dry Ice|
|Unit Size||50 µg|
|Resource Type||File Name||File Format|
|Application Note||How to Optimize a lyrosine Kinase Assay Using Time Resloved Fluorescence-based LANCE Detection||PDF 907 KB|
|Application Note||Measurement of p38/MAPK Activity Using LANCE||PDF 632 KB|
|Manual||LANCE generic reagents||PDF 101 KB|
|Technical Note||LANCE Ultra p60c-src Kinase Assay||PDF 469 KB|
|Poster||EGFR and human EphA4 receptor tyrosine kinaseassays for compound screening: Performance evaluation of LANCE®Ultra?TR-FRET assay platform||PDF 159 KB|