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LANCE Eu-W1024 Anti-phosphotyrosine (PY20), 1 mg

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in LANCE® TR-FRET kinase assays.

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Part Number Unit Size List Price Your Price Quantity
AD0066 50 µg 1013.00 USD
AD0067 1 mg 8400.00 USD
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Overview

The PY20 antibody (IgG2b) and PT66 antibody are mouse monoclonal antibodies that bind to phosphorylated tyrosine residues. Eu-W1024 labeled anti-phosphotyrosine PY20 antibody for LANCE® assays where the analyte concentration is < 1-10 nM and assay pH > 7. The PY20 antibody is a mouse monoclonal antibody, IgG2b, that binds to phosphorylated tyrosine residues.

Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates PY20
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
Product Brand Name LANCE
Shipping Condition Dry Ice
Unit Size 1 mg

Resources, Events & More

All (6)
Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note How to Optimize a lyrosine Kinase Assay Using Time Resloved Fluorescence-based LANCE Detection PDF  907 KB
Application Note Measurement of p38/MAPK Activity Using LANCE PDF  632 KB
Manual LANCE generic reagents PDF  101 KB
Technical Note LANCE Ultra p60c-src Kinase Assay PDF  469 KB
Poster EGFR and human EphA4 receptor tyrosine kinaseassays for compound screening: Performance evaluation of LANCE®Ultra?TR-FRET assay platform PDF  159 KB