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LANCE Eu-W1024 Anti-phosphotyrosine (P-Tyr-100), 10µg

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.

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Product Number Unit Size List Price Your Price Quantity
AD0203 10 µg 199.00 USD
AD0161 50 µg 983.00 USD
AD0162 1 mg 9200.00 USD

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Overview

Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates Anti-P-Tyr-100
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
Product Brand Name LANCE
Shipping Condition Blue Ice
Unit Size 10 µg

Resources, Events & More

All (6)
Resource Type File Name File Format
Application Note How to Optimize a Tyrosine Kinase Assay Using Time Resolved Fluorescence-Based LANCE™ Detection PDF 907 KB
Application Note Measurement of p38/MAPK Activity Using LANCE PDF 632 KB
Application Note Quench Correction for LANCE Time-Resolved Fluorescence Resonance Energy Transfer PDF 847 KB
Application Note Stability of the Wallac LANCE Eu-chelates PDF 579 KB
Manual LANCE generic reagents PDF 101 KB
Flyer PerkinElmer's Assay Services: Looking For a Non-Radioisotopic Serine Kinase Assay? PDF 117 KB