Europium-labeled anti-phospho-serine/threonine-proline antibody, for use in LANCE® TR-FRET kinase assays.
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Phospho-serine/threonine-proline antibody is a mouse monoclonal IgG antibody (supplied by Upstate). It recognizes a phosphorylated epitope found in phosphoproteins such as MAP2, HSP70, cdc25, and DNA topoisomerase Ila, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by the antibody varies from species to species and with cell type.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|Detection Method||TR-FRET, Time-Resolved Fluorescence (TRF)|
|Experimental Type||In vitro|
|Product Brand Name||LANCE|
|Shipping Condition||Dry Ice|
|Unit Size||10 µg|
|Resource Type||File Name||File Format|
|Application Note||How to Optimize a lyrosine Kinase Assay Using Time Resloved Fluorescence-based LANCE Detection||PDF 907 KB|
|Application Note||Measurement of p38/MAPK Activity Using LANCE||PDF 632 KB|