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LANCE Eu-W1024 phospho-(Ser) PKC substrate antibody, 10 µg

Europium-labeled anti-phospho-(Ser) PKC substrate antibody, for use in LANCE® TR-FRET kinase assays.

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AD0188 2970.00 USD

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Overview

Phospho-(Ser) PKC substrate antibody is an affinity-purified rabbit polyclonal antibody (supplied by Cell Signaling Technology). It detects phosphorylated serine residues with arginine or lysine at the -2 and +2 position, and a hydrophobic residue at the +1 position.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates Anti-P-Ser PKC
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
Product Brand Name LANCE
Shipping Condition Blue Ice
Unit Size 10 µg

Resources, Events & More

Resource Type File Name File Format
Application Note How to Optimize a lyrosine Kinase Assay Using Time Resloved Fluorescence-based LANCE Detection PDF 907 KB
Application Note Measurement of p38/MAPK Activity Using LANCE PDF 632 KB