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LANCE® Eu-W1024 phospho-serine antibody, 10 µg (Zymed)

Europium-labeled anti-phospho-serine antibody, for use in LANCE® TR-FRET kinase assays.

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AD0186 1503.00 USD

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Overview

Phospho-serine antibody is an affinity-purified rabbit polyclonal antibody (supplied by Zymed). It reacts specifically with proteins containing phosphorylated serine residues. Recognition of proteins containing phosphorylated serine by this antibody is independent of neighbouring amino acids and species of origin of the phosphorylated protein. This antibody is specific for phosphoserine-containing proteins and shows no significant cross-reactivity to proteins phosphorylated on threonine or tyrosine residues.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates Anti-P-Ser
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
Product Brand Name LANCE
Shipping Condition Dry Ice
Unit Size 10 µg

Resources, Events & More

Resource Type File Name File Format
Application Note How to Optimize a lyrosine Kinase Assay Using Time Resloved Fluorescence-based LANCE Detection PDF 907 KB
Application Note Measurement of p38/MAPK Activity Using LANCE PDF 632 KB