Europium-labeled anti-phospho-PKA substrate antibody, for use in LANCE® TR-FRET kinase assays.
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Phospho-PKA substrate antibody is an affinity-purified rabbit polyclonal antibody (supplied by Cell Signaling Technology). It is highly specific for phosphorylated threonine with arginine at the -3 position. It also recognizes phospho-serine with arginine at the -2 and -3 position.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|Detection Method||TR-FRET, Time-Resolved Fluorescence (TRF)|
|Experimental Type||In vitro|
|Product Brand Name||LANCE|
|Shipping Condition||Blue Ice|
|Unit Size||10 µg|
|Resource Type||File Name||File Format|
|Application Note||How to Optimize a lyrosine Kinase Assay Using Time Resloved Fluorescence-based LANCE Detection||PDF 907 KB|
|Application Note||Measurement of p38/MAPK Activity Using LANCE||PDF 632 KB|
|Manual||LANCE Eu-labeled antibodies for serine/threonine kinase assays||PDF 188 KB|